Shrimp alkaline phosphatase
SpletShrimp Alkaline Phosphatase (Product No. A2237) is supplied as a solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.6, 1 mM MgCl 2 and 0.1 mM ZnCl 2. Dilutions can be … SpletDESCRIPTION biotechrabbit™ Shrimp Alkaline Phosphatase is a multipurpose alkaline phosphatase that dephosphorylates most biomolecules and is fully inactivated by heating for 5 min at 65°C. The enzyme is stable at room temperature and active in common buffers for restriction digestion and PCR.
Shrimp alkaline phosphatase
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http://www.haigene.cn/html/show-324.html SpletShrimp Alkaline Phosphatase Shrimp ALKP is a dimeric protein with a molecular weight of approximately 58 kDa as determined by SDS-PAGE. The Shrimp enzyme denatures at a …
SpletAlkaline phosphatases (APs) are homodimeric metalloenzymes that catalyze the hydrolysis and transphosphorylation of phosphate monoesters. Each monomer contains a metal-binding triad that for optimal activity is usually occupied by two zinc ions and one magnesium ion. SpletShrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.
http://korambiotech.com/upload/bbs/2/m0371-shrimp-alkaline-phosphatase-rsap.pdf SpletShrimp alkaline phosphatase (SAP) has a high specific activity and is a heat-labile alkaline phosphatase. It can be inactivated by a short rise in temperature to 65 °C. SAP is a …
Splet19. okt. 2001 · Alkaline phosphatases are non-specific phosphomonoesterases that are distributed widely in species ranging from bacteria to man. This study has concentrated …
Splet25. dec. 2001 · The gene encoding alkaline phosphatase (AP) from the psychrophilic strain TAB5 was cloned, and its nucleotide sequence was determined. A single open reading frame consisting of 1125 base pairs which encodes a polypeptide consisting of signal peptide of 22 amino acids and a mature protein of 353 amino acids was identified. fieldturf commercialSpletShrimp alkaline phosphatase has been used to degrade primers from amplified samples after PCR. Features and Benefits Removing 5′-phosphates from DNA and RNA. Easily … grichka and igor plastic surgerySpletShrimp Alkaline Phosphatase (SAP) is a high specific activity, heat-labile alkaline phosphatase purified from a recombinant source and originally isolated from Pandalus borealis (arctic shrimp). SAP is useful in many molecular biology applications such as the dephosphorylation of phosphorylated ends of DNA or RNA for subsequent use in cloning ... grichka and igor bogdanoff photosSpletShrimp Alkaline Phosphatase (SAP) – still the golden standard and the first heat-labile alkaline phosphatase on the market. It is a multipurpose alkaline phosphatase that can be fully inactivated by a short heat treatment. 100% heat-inactivated at 65°C Works in restriction enzyme buffers Used in PCR clean-up fieldturf colorsSpletShrimp Alkaline Phosphatase (rSAP)はアルカリホスファターゼであり、CIPと異なって熱による不活性化ができる。 タグや修飾が無いリコンビナント酵素であり、野生型酵素 … grichka bogdanoff 1980SpletDie Shrimp Alkaline Phosphatase ( SAP) ist ein aus Eismeergarnelen ( Pandalus borealis) isoliertes Enzym, das in der Molekularbiologie häufig Verwendung findet. Als alkalische … grichka and igor bogdanoff redditSpletShrimp alkaline phosphatase (recombinant), (rSAP) is a multipurpose alkaline phosphatase that is fully inactivated by a short heat treatment. rSAP has all the properties of the native enzyme, but with the additional benefits of greater stability, consistency, and purity. rSAP simplifies cloning and sequencing workflows due to a complete heat ... grichka bogdanoff 1979