Pre-chilled pbs
WebPre Chilled Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebApr 19, 2024 · The mucus was gently scraped with a slide on an ice bath. Then the mucus was combined and homogenized in pre-chilled PBS, and was centrifuged (5000 r/min, 30 …
Pre-chilled pbs
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WebSep 6, 2024 · The cells were then spinned down at 2000 rpm for 5 min and resuspended in pre-chilled PBS buffer at 2 × 10 5 cells/mL density. Cells were stained with Armenian … WebMay 7, 2024 · The pellet was homogenized with Tissue Lyser II (Qiagen, Valencia, CA, USA) in 2 mL of pre-chilled PBS buffer (pH 7.5) with glass beads in the pre-chilled Tissue Lyser adaptors, the supernatant was colored by mixing with Bradford reagent (Sigma Chemical Co., St. Louis, MO, USA), and The quantitative amount of protein of each sample was …
WebAug 17, 2024 · The NR viability assay kit (Suzhou Kemin Biological Co., Ltd., item no. NR-2-Y) was used for NR viability determination. One hundred milligrams of material was homogenized by grinding in 1 mL of pre-chilled PBS (50 mmol/L, pH 7.0) buffer in an ice bath and centrifuged at 8000 rpm for 10 min at 4℃. WebSep 6, 2024 · The cells were then spinned down at 2000 rpm for 5 min and resuspended in pre-chilled PBS buffer at 2 × 10 5 cells/mL density. Cells were stained with Armenian hamster anti-CD29 (1:500 ...
WebJul 24, 2015 · Testes were cleaned in pre-chilled PBS on ice and weighed using balance (CPA225D, Sartorius, Göttingen). Testes homogenates were prepared in pre-chilled PBS … WebJan 10, 2024 · BV2 cells were inoculated into the 6-well plates and treated with LPS/IFN-γ for a day. After rinsing with pre-chilled PBS twice, the DCFH-DA probe was diluted with serum-free medium at 1:1000, the DHE probe was diluted at 1:1500, and 1 ml diluted fluorescence probe solution was added into each well to deeply incubate the cells for 30 min.
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WebJul 24, 2015 · Testes were cleaned in pre-chilled PBS on ice and weighed using balance (CPA225D, Sartorius, Göttingen). Testes homogenates were prepared in pre-chilled PBS (10 % w/v) by tissue homogenizer (OMNI, TH, USA) and centrifuged with 12000 g at 4 °C for 15 min. Supernatants were stored immediately at -80 °C for further analysis, if not used on … egg drop soup good for youWebNote: Pre-chill an appropriate volume of RIPA Lysis Buffer at 4°C. If desired, add protease inhibitor and phosphatase inhibitor to the lysis buffer immediately before use. 1. Place the … egg drop soup with bean sproutsWebHeLa cells were treated with 45 (5 μM) at 37°C for 1 h, pre-chilled at 4°C for 1 h, then infected with PRV (50 MOI) at 4°C for 1 h. After that, cells were washed with pre-chilled PBS and transferred into the incubator to allow viral entry. After 1 h, cells were fixed and analyzed by indirect immunofluorescence. foldable chinese lanternWebJun 25, 2024 · The dried lipids were hydrated with 250 µl pre-chilled PBS buffer in the range of 2–10 mg/ml and sonicated in a water bath for 30 min, maintaining a temperature of ~ 4 °C. foldable chest press benchWebFeb 11, 2024 · Add 0.2 volume of chloroform per 1 volume of TRIzol reagent. Cap the tubes securely and vortex samples vigorously for 15 seconds. Incubate samples at room temperature for 5 minutes. Centrifuge the samples at no more than 12,000 x g for 15 minutes at 4°C. The mixture will separate into three phases (Figure 1). egg drop soup chinese recipeWeb12. Centrifuge 14,000g for 5s, keep the pellet and wash with pre-chilled washing buffer (or cold PBS) for 3 times. (800μl each) 13. Collect the supernatant to proceed to SDS-PAGE, … egg drop soup souped up recipesWebJan 25, 2013 · Cells were then washed with 50 µl/well of pre-chilled PBS three times, incubated in 50 µl of pre-warmed DMEM and kept for 1 h at 37°C. (2b) To investigate the … foldable child toothbrush