WebJun 1, 2024 · A palmitoylated RGS4 standard (Palm-RGS4-Std) is prepared by incubating purified RGS4 with palmitoyl-CoA under conditions that promote autopalmitoylation of RGS4 at cysteine residues previously mapped as palmitoylation sites [ 24 ]. The same protocol should be applicable toward the analysis of proteins with other forms of S -acylation. 2 … WebDetection of Human IFN‑ alpha / beta R1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human IFN‑ alpha / beta R1 Monoclonal Antibody (Catalog # MAB2452) followed by HRP-conjugated Anti-Rabbit ...
Direct Analysis of Protein S-Acylation by Mass Spectrometry
WebFeb 18, 2013 · This is of particular interest in neurons where many synaptic proteins are palmitoylated. We utilize a simple biochemical method to detect palmitoylated proteins in cultured neurons, which can be adapted for multiple cell types and tissues. ARTICLE ADD TO PLAYLIST USAGE STATS 29,460 Web1 day ago · S-Palmitoylation of SERT. (A) Detection of S-palmitoylated SERT by acyl-RAC. AD293 cells were transfected with FLAG-SERT and then cultured for 12–48 h. S-Palmitoylated FLAG-SERT was detected by acyl-RAC. Omission of NH 2 OH served as a negative control. Data are representative of three independent experiments. mean magnitude of relative error
Palmitoylation of the KATP channel Kir6.2 subunit promotes …
WebThere is no information on the scope of protein S-palmitoylation, or the pattern of DHHC enzyme expression, in the heart. We used resin-assisted capture to pull down S-palmitoylated proteins from human, dog, and rat hearts, followed by proteomic search to identify proteins in the pulldowns. WebJun 21, 2007 · The identified PPs encompassed all the known types of PPs—proteins that tether to membranes solely through palmitoylation, proteins that are palmitoylated in … WebJun 8, 2024 · Moreover, palmitoylation is required for Tat to accumulate at the plasma membrane and affect PI(4,5)P2-dependent membrane traffic such as phagocytosis and neurosecretion. ... Detection of ... pearson fees 2021/22