WebMEGAshortscript T7 Transcription Kit (Thermo Fisher Scientific AM1354) MgCl2(500 µm) NEBuffer2 (NEB B7002S) PCR master mix (2×; see Table 1) PCR primers designed to amplify 600–800 bp containing the CRISPR target … WebAmbion, Inc. Improved High Yield Transcription The patented MEGAshortscript™ High Yield Transcrip-tion Kit has been improved with the addition of SUPERase-In,™ a broad …
In Vitro Reconstitution a SARS-Coronavirus mRNA Cap Methylation
WebThen use T7 megascript kit from Ambion (for short sequences, the megashortscript kit is also good). So : - PCR reaction and purification in RNAse-free conditions - T7 … Web27 mei 2016 · Section snippets Cas9/sgRNA production. Recombinant Cas9 protein was purchased from PNABio. In vitro transcription and purification of sgRNAs were performed … toolstation 6 way consumer unit
WO2024031934A1 - Agents that target the glycine arginine-rich …
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