NettetWe describe a flow cytometry (FCM) technique to monitor the MDR phenotype in drug resistant variants of SCLC and non-SCLC cell lines. The technique is based on a multiparametric analysis of the nuclear binding of a model chemotherapeutic agent, the fluorescent dye Hoechst 33342 (Ho342), which is capable of supra-vital staining of … Nettet21. des. 2015 · ReprodFert 1990; 88:343-352. 18. Harrison RAP, Mairet MillerNGA. Flow cytometric studies bicarbonate-mediatedCa boarsperm populations. Mol Reprod Develop 1993;35: 197-208. 19. Henley RobertsKD. Flow cytometric evaluation acrosomereaction humanspermatozoa: newmethod using photoactivatedsupravital stain. Intern …
PureBlu Hoechst 33342 Nuclear Staining Dye - Bio-Rad …
NettetThe properties of a fluorochrome will depend on its environment. For example, propidium iodide (PI), which is used to stain DNA, is only weakly fluorescent in water; on intercalating with DNA, the fluorescence increases 50 fold due to the hydrophobic environment. Some fluorochromes, such as fluorescein, are sensitive to pH. Nettet9. feb. 2013 · Flow cytometry analysis. A : the apoptosis ratios of MGC-803 cells treated with BTA and OEA (20 μ M) assessed by flow cytometry. B : flow cytometry analysis for apoptosis inducing activities of BTA and OEA on MGC-803 cells, a: control; b, c, and d: treated with BTA (20 μ M); e, f, and g: treated with OEA (20 μ M) braehead centre
Hoechst 33342 DNA staining Instruction & Forms - U-M …
Nettet3. mai 2024 · This method used Hoechst 33342 to stain the spermatozoa and sorted the X and Y chromosome-bearing spermatozoa using flow cytometry. The sexed sperm are subjected to in vitro fertilization and subsequent artificial insemination in cattle and pigs (Johnson et al., 1999 ). 4.6 Applications of Sexed Semen Sorted by FACS 4.6.1 Cattle Nettet17. aug. 2015 · Hoechst is a dna stain typically used to identify cell cycle profiles in live cells. It can and will excite with both a UV and a strong violet laser and will read in a det'r with a 450/50BP... NettetWe describe a flow cytometry (FCM) technique to monitor the MDR phenotype in drug resistant variants of SCLC and non-SCLC cell lines. The technique is based on a … hackerman template