Web5 dic 2015 · Immunofluorescence. differenttimes posttransfection, cells were washed twice phosphate-bufferedsaline (PBS) lacking Ca 20min methanol-acetone(1:1) cellswere air dried fewminutes roomtemperature prior primarymouse antibody, J2 monoclonal antibody (MAb J2; Engscicons), which directedagainst double-stranded RNA (dsRNA) longer than 40 … dsRNA (J2) Mouse mAb recognizes dsRNA where the length of the helix is greater than or equal to 40 base pairs (bp). The ability to recognize dsRNA is independent of the sequence and nucleotide composition of the antigen. The J2 clone shows a higher affinity to natural dsRNA than to other dsRNA antigens (6).

Anti-dsRNA monoclonal antibody J2 - Jena Bioscience

WebThe J2 anti-dsRNA IgG2a monoclonal antibody has become the gold standard in dsRNA detection. It was used initially for the study of plant viruses, but since the seminal paper of Weber et al. in 2006, where J2 was used to show that all the positive strand RNA viruses tested produced copious amounts of dsRNA in infected cells, ... WebMouse monoclonal antibody J2 recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent … motorcycle shop yuma az

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Web11 mar 2024 · Double-stranded RNA (dsRNA) is a virus-encoded signature capable of triggering intracellular Rig-like receptors (RLR) to activate antiviral signaling, but whether … WebAlternative Name(s) of Target: double-stranded RNA Immunogen: The original J2 antibody was raised by immunising mice with L-dsRNA. Specificity: This antibody binds to dsRNA > 40 bp in length. Application Notes: This recombinant mouse monoclonal antibody, rJ2, is useful for detecting double stranded RNA of greater than 40 bp in length which is … WebJournal: BMC Cancer Article Title: Replicase-based plasmid DNA shows anti-tumor activity doi: 10.1186/1471-2407-11-110 Article Snippet: Monoclonal anti-dsRNA antibody J2 (English & Scientific Consulting Bt. Techniques: Transfection, Reverse Transcription Polymerase Chain Reaction, Amplification, Enzyme-linked Immunosorbent Assay, … motorcycle shop wymondham