2024 Cst atf4 antibody

Cst atf4 antibody

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August undefined, 2024

WebJun 24, 2024 · PERK IPs were performed with 1 mg of lysate incubated with 2 µg of primary antibody (Proteintech, #20582-1AP) or the corresponding IgG (Cell Signaling Technology, #2729) overnight at 4 °C with ... WebImmunoprecipitation of ATF-4 from extracts of 293 cells, treated with tunicamycin (2 μg/ml, 8 hr), using Rabbit (DA1E) mAb IgG XP ® Isotype …

Isoproterenol Increases RANKL Expression in a ATF4/NFATc1 …

Webrepresentative of CST, are rejected and are of no force or effect. P roduc t s are labeled wit h For Res earc h Us e O nly or a s imilar labeling s t at ement and hav e not been approv ed, c leared, or lic ens ed by t he FDA or ot her regulat ory f oreign or domes t … WebMar 3, 2024 · Effective treatments for patients suffering from heat hypersensitivity are lacking, mostly due to our limited understanding of the pathogenic mechanisms underlying this disorder. In the nervous system, activating transcription factor 4 (ATF4) is involved in the regulation of synaptic plasticity and … readsoft helpful tips tricks

ATF4/ATG5 Signaling in Hypothalamic Proopiomelanocortin Neurons ...

Web(11815) ATF-4 (D4B8) Rabbit mAb - Cell Signaling Technology - CiteAb. 670 citations – A rabbit monoclonal antibody, raised against Cyclic AMP-dependent transcription factor ATF-4 (Human), supplied by Cell Signaling Technology. ... ATF4, Activating transcription factor 4, CREB-2, CREB2, Cyclic AMP-dependent transcription factor ATF-4, Cyclic ... WebMonoclonal Antibody for studying ATF-4. Cited in 3 publications. Validated for Western Blotting, Immunofluorescence (Immunocytochemistry). Highly specific and rigorously … WebThis antibody has been shown to work in applications such as: Precipitation, Sequencing, ChIP-Seq, ChIP, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot, and Other. ATF-4 (D4B8) Rabbit … readsoft forms 5.3

Central Activating Transcription Factor 4 (ATF4) Regulates …

WebNov 1, 2024 · Membranes were then incubated at 4 °C overnight with ATF4 antibody (BioLegend) at 1:1000. After washing with 1x TBST, anti-rat HRP secondary antibody (CST) was used at 1:2000. A list of commercially available antibodies tested in livers and cell lines is included in Supplementary Table 1. 2.6. Gene expression analysis. WebHowever, CS-11815 detects a band with a mass of ~49 kDa, while the expected molecular weight of ATF4 is ~38 kDa. In contrast, SC-200 is a very dirty polyclonal antibody, but it seems to detect ... readshuttleWebMar 25, 2024 · Some oncoproteins along with stress kinase general control non-derepressible 2 (GCN2) can ensure the induction of activating transcription factor 4 (ATF4) to counteract amino acid deprivation; however, little is known regarding the role of the oncogenic EGFR-PI3K pathway. In this study, we demonstrate that both mutated EGFR … how to tab through fields in word

"WebMay 5, 2024 · About 500 mg protein of total cell lysates was incubated with primary antibody anti-ATF4 (#11815, CST or 10835-1-ap, Proteintech group, USA) or anti-CTCF (#3418, CST or sc271514, Santa Cruz Biotechnology, USA) at 4 °C with overnight shaking. The immune complexes were then incubated with Protein A/G Dynabeads (Thermo … " - Cst atf4 antibody

Cst atf4 antibody

Anti-ATF4 Antibody [S360A-24] Monoclonal IgG2a

WebMar 3, 2024 · In the nervous system, activating transcription factor 4 (ATF4) is involved in the regulation of synaptic plasticity and memory formation. Here, we show that ATF4 … WebOct 22, 2010 · ATF4 is a transcriptional activator of the integrated stress response, a program of gene expression involved in metabolism, nutrient uptake, anti-oxidation, and the activation of additional transcription factors, such as CHOP/GADD153, that can induce apoptosis. ... (Cell Signaling Technologies). ATF4 antibody was prepared against …

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Web1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF4 (60035-1-Ig, Clone:3E4C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at dilution 1:1000 (red), or 0.4 ug Control … WebThis antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 38 kDa ATF4 protein and its phosphorylated …

WebThe antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these …

WebATF4 Antibody - Mouse monoclonal antibody to human Activating Transcription Factor 4, clone S360A-24. Validated for WB, IHC, ICC/IF with human, rat tissue. ... Cell Signaling, Epigenetics and Nuclear Signaling, Neuroscience : Cellular Localization : Cell membrane, Cytoplasm, Nucleus : Accession Number : NP_001666.2 ... WebAug 13, 2024 · This was especially relevant as we found in the public CHIP-seq data base (chip-atlas.org), that the Nrf2 antibody bound to the ATF4 promoter (~1 kB from the TSS) in the cardiovascular HAEC cell ...

WebJan 10, 2024 · Moreover, ATF4 overexpression inhibited tumor proliferation and AR expression both in vitro and in vivo. Collectively, these results demonstrated that ER …

WebActivating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor … how to tab through open windowsWebMar 21, 2024 · CHST4 (Carbohydrate Sulfotransferase 4) is a Protein Coding gene. Diseases associated with CHST4 include Mucinous Adenocarcinoma and Colon … how to tab within an excel cellWebDec 25, 2024 · The membrane was blocked and then incubated overnight at 4°C with primary antibody as described previously ... antibody to phospho-T389 p70S6K1 was obtained from Cell Signaling Technology (catalog number: 9205); antibodies to REDD1 (catalog number: 10638-1-AP), Sestrin1 (catalog number: 21668-1-AP), and Sestrin2 … how to tab with apple pencilWebSep 29, 2024 · ATF-4 Antibody (B-3) is an IgG 2b κ mouse monoclonal ATF-4 antibody (also designated ATF4 antibody, cAMP-dependent transcription factor ATF-4 antibody, CREB-2 antibody or cAMP … how to tab through windowsWebATF4 Rabbit pAb (A0201) Western blot analysis of extracts of HeLa cells, using ATF4 antibody (A0201) at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/mL) for 8 hours. Secondary antibody: HRP Goat Anti … readsiWebJun 14, 2013 · The transcription factor activating transcription factor 4 (ATF4) belongs to the cAMP-responsive element–binding protein family, characterized by the presence of a leucine zipper dimerization domain and a basic amino acid–rich DNA binding domain ().Previous studies have shown that knocking out ATF4 gene expression in mice results … how to tabulate data in stataWebMonoclonal Antibody for studying ATF-4. Cited in 661 publications. Validated for WB, IP, IF, ChIP, ChIP, C&R. Highly specific and rigorously … readsoft ap automation