Click-it edu kit
WebThe Click-iT® EdU Flow Cytometry Assay Kits are novel alternatives to the BrdU assay. EdU (5-ethynyl-2´-deoxyuridine) is a nucleoside analog to thymidine and is incorporated … WebThermo Fisher Scientific - US
Click-it edu kit
Did you know?
WebClick-iT EdU 技术的优势在于化学方面 - 独特的小分子和低背景的标记物以及检测基团之间特异性共价反应。 5-乙炔基-2'-脱氧尿苷（EdU）是含有炔烃的核苷类似物。 在铜催化 … WebC0081L. BeyoClick™ EdU-647细胞增殖检测试剂盒. 200-2000次. 2183.00元. BeyoClick™ EdU-647细胞增殖检测试剂盒 (BeyoClick™ EdU Cell Proliferation Kit with Alexa Fluor 647)，是一种基于DNA合成过程中胸腺嘧啶脱氧核苷 (thymidine)类似物EdU (5-ethynyl-2' -deoxyuridine)的掺入，并通过随后的点击 ...
WebThe Click-iT EdU cell proliferation assay (Invitrogen) for detection of replicating cells is based on incorporation of EdU into newly synthesized DNA and its recognition by azide dyes via a copper mediated "click" reaction. In the protocol provided by Invitrogen, cells are fixed with paraformaldehyde and stained with 7-aminoactinomycin D (7-AAD ... WebFeb 19, 2016 · Asghar - We have used the Click-It Edu kit from Invitrogen (Thermo). After the Click reaction, we have done Hoescht 33342 staining and standard immunocytochemistry without any issues.
WebJan 31, 2024 · Mix to dissolve. Adjust pH to 6.0 with 1N HCl and then add 0.5 ml of Tween 20 and mix well. Store this solution at room temperature for 3 months or at 4 C for longer storage. Note: this buffer is ...
WebClick-iT® EdU Microplate Assay The Click-iT® EdU microplate assay protocol differs from the Click-iT® EdU assays for fluorescence microscopy, flow cytometry, or high-throughput imaging. ... Caution DMSO (Component C), provided as a solvent in this kit, is known to facilitate the entry of organic molecules into tissues. Handle reagents ...
WebMar 9, 2015 · ®1.5 To make a 10X stock solution of the Click-iT EdU buffer additive (Component G), add 2 mL of deionized water to the vial and mix until the Click-iT® EdU buffer additive is fully dissolved. After use, store any remaining stock solution at ≤–20˚C. When stored as directed, the stock solution is stable for up to 1 year. folding glass door revit familyWebFeb 15, 2009 · EdU chemistry. The 50 assay Click-iT™ EdU Cell Proliferation Assay Kit (Cat# C35002, Invitrogen) contains 12 reagents named component A to component L. Components A, B, E, G, H and I are required. ... to detect GLIDR expression in glioma cells. Cell counting kit 8 (CCK-8) assay, colony formation assay, JC-1 staining, and flow … folding glass door for balconyWebFeb 15, 2009 · An alternative thymidine analogue is 5-ethynyl-2′-deoxyuridine (EdU), in which the terminal methyl group is replaced with an alkyne group, which allows detection using a fluorescent azide that covalently binds to the alkyne group in a reaction known as “click chemistry” (Hsu et al., 2007, Sawa et al., 2006, Tornoe et al., 2002) (Fig. 1).This … folding glass doors manufacturerWebmethod. In contrast, the Click-iT® Plus EdU cell proliferation kit is compatible with cell cycle dyes, R-PE, R-PE tandems, and fluorescent proteins such as GFP, RFP, and mCherry. Figure 2 below demonstrates Click-iT® Plus EdU multiplexed with Hu CD3 PE and Figure 3 (page 3) shows Click-iT® Plus EdU multiplexed with FxCycle™ violet and GFP. folding glass doors exterior priceWebEdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. With Click-iT® EdU, mild fixation and detergent permeabilization is sufficient for the small molecule-based Click-iT® EdU detection reagent to gain access to the DNA. As a consequence, the Click-iT® EdU HCS assay is ... egret white wallsWebThe Click-iT EdU Alexa Fluor 555 Imaging Kit provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine and is incorporated … folding glass doors patioWebwahoo.nsm.umass.edu egrf0811l30d1wh-148a