Web20 hours ago · Non-coding RNAs (ncRNAs) can control the flux of genetic information; affect RNA stability and play crucial roles in mediating epigenetic modifications. A number of studies have highlighted the potential roles of both virus-encoded and host-encoded ncRNAs in viral infections, transmission and therapeutics. However, the role of an … WebFeb 14, 2024 · Three pieces of software, circseq_cup, CIRI-full, and CircAST, were used for reconstruction of full-length sequences of circRNA with default parameters. Among them, CIRI-full and CircAST both require information of identified circRNA and sequencing reads as input, while circseq_cup only needs sequencing reads as input.
CIRCprimerXL: Convenient and High-Throughput PCR Primer …
WebCircular RNAs (circRNAs) are a recently discovered class of RNAs derived from protein-coding genes that have important biological and pathological roles. They are formed through backsplicing during co-transcriptional alternative splicing; however, the unified mechanism that accounts for backsplicing decisions remains unclear. Factors that regulate the … WebJan 15, 2024 · CircRNAs contain microRNA (miRNA) binding sites and can function as miRNA sponges to regulate gene expression by removing the inhibitory effect of an miRNA on its target gene. Methods:We used the bioinformatics software TargetScan and miRanda to predict circRNA-miRNA and miRNAi-Mrna interactions. glow captions
Circular RNA Expression: Its Potential Regulation and Function
WebApr 1, 2016 · DCC applies a series of filters and integrates data across replicate sets to arrive at a precise list of circRNA candidates. We assessed the detection performance of DCC on a newly generated mouse brain data set and publicly available sequencing data. WebArraystar Circular RNA Arrays, systematically profiling the expression of circular RNAs, are the only commercially available tool to most effectively profile, analyze and explore this new and exciting world of noncoding RNAs. Synthesis of circRNAs via intron-assisted splicing and RNaseR digestion has been previously described4, but rapid creation of different circRNA species was difficult. To enable higher-throughput testing of circRNAs, we created a modular cloning platform consisting of a set of backbones and parts in a clearly … See more We previously showed that circRNAs can trigger immune responses in vivo that can be avoided by modifying circRNAs with m6A4,11. However, … See more 5′ and 3′ UTRs in mRNAs can recruit RNA-binding proteins (RBPs) that enable strong translation as well as aspects of post-transcriptional regulation6. One such family of RBPs is … See more We first sought to uncover principles behind circRNA vector topology that are necessary for strong translation. We began by synthesizing circRNAs with a coxsackievirus B3 … See more Viral IRESs are diverse and highly structured RNA regions found primarily in viral 5′ UTRs that promote cap-independent translation30,31,32. Because iCVB3, the baseline … See more glow bib shorts m