Bnl cl.2
WebMar 8, 2024 · The effects of SEV on the miR-124-3p/TRAF3/CREB axis were also verified in vitro and in vivo. IP assay was performed to verify the effect of TRAF3 on CREB ubiquitination in BNL CL.2 cells, and the cycloheximide (CHX) intervention experiment to detect the stability of CREB protein. SEV augmented the miR-124-3p expression in I/R … WebSep 28, 2024 · The murine hepatocyte cell line BNL CL.2 (CL2) was irradiated with 10 Gy. Irradiation activated p53, induced downstream p21 in the liver, and delayed liver regeneration after PHx. While PHx increased hepatocyte growth factor (HGF) levels and activated Met with or without irradiation in the regenerative liver, it activated Akt and …
Bnl cl.2
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WebFeb 3, 2024 · The viral supernatant was applied directly on BNL CL.2 cells (for Tff3-, Igfbp5-) and human liver tumor HepG2 cells (for hTFF3-) for knockdown tests. Polybrene was added (1–10 μg/mL) to enhance infection efficiency. Target cells were selected using puromycin (1–10 μg/mL) and expanded or harvested for the preparation of whole-cell … WebJun 25, 2024 · BNL CL.2 cells were infected with three rounds of viral supernatants and selected with hygromycin (75 μg/ml) or puromycin (3 μg/ml). For amino acid starvation with or without bafilomycin A1 (BafA1) treatment, HEK293T cells in culture dishes were rinsed with PBS and incubated in serum and amino acid-free RPMI media for 48 h with BafA1 …
WebCharacteristics. No; No, the cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. The line was selected to grow in medium containing … WebMouse Normal Liver Cell Line Bnl Cl 2, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, …
WebNo production by BNL CL.2 cells was induced by interferon-r (IFN-r) plus lipopolysaccharide (LPS). However, other inflammatory cytokines such as interleukin (IL)-beta, tumor necrosis factor alpha (TNF-alpha), and IL-6 had no additional effects on it. The stimulatory effects of IFN-r and LPS were time- and dose-dependent. NO secretion was ... WebJan 15, 2005 · BNL SV A.8 cells were more sensitive to RCMF-mediated cytotoxicity than were MPHC or BNL CL.2. RCMF-mediated reduction of MnSOD activity and glutathione (GSH) content in BNL SV A.8 cells is thought to be associated with RCMF-induced apoptosis. Our findings suggest that RCMF is an agent which may be capable of …
WebWe have focused on elucidating factors that induce EMT to promote carcinogenesis and subsequent metastasis in HCC using the BNL CL.2 (BNL) and BNL 1ME A. 7R.1 (1MEA) cell lines. BNL cells are normal hepatocytes whereas the 1MEA cells are HCC cells derived from chemical transformation of the BNL cells.
WebTo cite this cell line use: BNL CL.2 (RRID:CVCL_4383) Comments. Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981). Derived from … dr sherwood rheumatologist virginia beachWebA–C, BNL CL.2 cells expressing Ifit1 were or were not treated with TNF-α and actinomycin D (ActD) for 5 hours. A, Coimmunoprecipitation with an anti-Flag antibody or control immunoglobulin G ... color foil nail polish sterling silverWebbnl cl.2 (cl2) Cells Products Every step of the way, a wide range of cell health products Maintaining healthy cells is the key to experimental success and reproducible research … dr shery cherianWebMay 19, 2010 · BNL CL.2 cells (1 × 10 5) were seeded in 6-cm 2 dishes and infected with retrovirus carrying SV40 large T antigen or treated with NMU for 24 hours. After washing three times with PBS, the retrovirus-infected cells were cultured in the growth medium containing puromycin (1 μg/ml) for 2 weeks. color foil nail polish sally hansenWebAdd 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Ratio: 1:2 to 1:4. … color font text copy and pasteWebThis cell line is the high passage culture of the original mouse liver cell line BNL CL.2 (ATCC® TIB-73™). This cell line is to be used as a control for F4 G6PD Knockout cell … dr shery goril niagara falls nyWebFeb 1, 2008 · In generating aptamers for liver cancer recognition, two liver cell lines were used: a liver cancer cell line BNL 1ME A.7R.1 (MEAR) and a noncancer cell line, BNL CL.2 (BNL). ... BNL CL.2 (BNL). Both cell lines were originally derived from Balb/cJ mice. Through multiple rounds of selection using BNL as a control, we have identified a panel … dr. sheryl anderson columbia md